News on Photolysis

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News on Photolysis

Time Table

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Lectures Schedule - Open to scientists

Demonstration for registered students only

Time Table

PARIS NEUROSCIENCE SCHOOL

Photolysis - News on Photolysis

LECTURE AND DEMONSTRATION  TIMETABLE
Tues 20 April

9.30 Welcome and Introductory information
11.00 Boris Barbour Electronics of patch clamp recording
14.00 Alain Marty Whole cell patch clamp
15.30 Sandrine Leveque-Fort Molecular Photophysics
17.00 Larry Cohen Membrane potential indicators
Weds 21 April

9.00 Martin Thomas Light sources
11.00 Gail McConnell Lasers for one and two photon excitation
13.30 Klaus Suhling Detectors and CCD cameras
15.00 Brad Amos Transmitted light microscopy and contrast methods
16.30 Stefanie Reichelt Scanning laser confocal and multi- beam microscopy
Thurs 22

9-00 Jean Livet The Brainbow mouse

Microscopy Demonstrations
18.00 Hubert Affolter The HEKA patch clamps: software control and data acquisition.
Fri 23 April

9.00 Clement Lena Extracellular recording

Microscopy Demonstrations
18.00 Christophe Pouzat Spike train analysis. Statistical methods in data analysis
Sat 24 April

9.30 Marco Beato Single channel kinetics underlying synaptic currents.
18.00 Isabel Llano Fluorescent indicators of ion concentration
Mon 26 April

9.00 Laurent Moreaux Two photon excitation and non-linear optical techniques

Demonstrations at ENS
18.00 Lyle Graham Dynamic clamp of membrane conductance.
Tues 27 April

11.30 Troy Margrie In vivo patch clamp and imaging

Demonstration

In vivo patch clamp
18.00 Stephane Dieudonne Fast multipoint confocal imaging
Weds 28 April

9.00 Martin Oheim Design of a two photon confocal microscope

Demonstration In Vivo patch clamp
18.00 David Ogden Photolysis
Thurs 29 April

9.00 Valentina Emiliani Holographic methods in microscopy

Demonstrations
Martin/Nicole/Valentina-Cathie
6-00 Maxime Dahan Single molecule detection
19.30 Dinner
Fri 30 April

9.00 Uwe Maskos Transfection with viruses.

Demonstrations – Viral transfection Jonathan Bradley

General arrangements.

The first two days will be lectures only with some time for finding out about Paris and local labs. From Thursday 22nd between 11-00 and 6-00 there will be 6 practical experiments with electrophysiological recording and imaging, and demonstrations in local labs.

Demonstrations will be after lunch. Some demonstrations will be restricted to about 6 people so a sign-up system will allow selection, each person attending 2 or 3 of those available. This arrangement is not finalised and is flexible, but some demos will not accommodate more than a few people at a time. Where they involve introductory talks these can be open for all interested in the teaching classroom. The topics are indicated in the timetable.

The practical experiments have the following general topics
  • Extracellular spike recording and analysis
  • Patch clamp recording and stimulation in slices.
  • CCD imaging of nerve tract excitation and postsynaptic recording
  • Voltage sensitive indicators of membrane potential
  • Photolysis
  • Whole cell recording and CCD imaging of Ca concentration
  • Dendritic patch clamp recording
Each will be available to in 3 rotations of 2-3 days to groups of 2-4 students.

Lunch and coffee breaks will be in the common room adjacent to the practical classroom.


   

SUMMER SCHOOL PARIS 2010

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OPTICAL IMAGING AND ELECTROPHYSIOLOGICAL RECORDING IN NEUROSCIENCE

Summer School of the Ecole Neuroscience Paris - Ile de France

April 20 – April 30 2010

Université Paris Descartes – Rue des Saints Pères 75006 PARIS

   

Information for Applicants

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The Paris School in Neuroscience aims to give intensive tuition in the technical basis and practical implementation of optical imaging and electrophysiological recording in the the Neurosciences. The topics covered in lecture and practical work are:

  • Photophysics and photochemistry of fluorophores.
  • Light sources and lasers for fluorescence excitation.
  • Conventional and confocal microscopy
  • CCD cameras
  • Patch clamp and extracellular recording. Multi-electrode arrays. In vivo electrical recording.
  • Data analysis and image analysis.
  • Two-photon confocal and other non-linear microscopy
  • Fluorescent indicators for calcium and other ions and membrane potential
  • Adaptive optics in microscopy
  • Photolysis
  • Brain slice and In vivo imaging
  • Viral transfection of genetically encoded markers and indicators.

The lectures are at 9-00 and 6-00 each day before and after daily practical classes.

Practical work will be 1-3 day experiments in one of the above topics.

The School fee is 800 Euro

Accommodation (for 10 nights) is close to the University and includes breakfast, lunch is provided in the lab each day. The fee of 800 euro includes accommodation. Participants are responsible for their own travel arrangements.

How To Apply: To register: click on the link

There are 18 places. Applications should be made on the website and must include a brief (1-2 page ) CV, description of the current research project and a letter of recommendation form advisor, lab head or a senior colleague. Some bursary support as a half fee will be available.

REGISTRATION IS CLOSED FOR 2010

   

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